cd43 b cells (Miltenyi Biotec)
Miltenyi Biotec is a verified supplier 
Miltenyi Biotec manufactures this product 
96
Structured Review
Miltenyi Biotec
cd43 b cells
Cd43 B Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd43+b+cells/CD43+Glycoform+Antibody%2C+anti-mouse%2C+REAfinity/pm42001515-35-4-24
Average 96 stars, based on 1 article reviews
Cd43 B Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd43+b+cells/CD43+Glycoform+Antibody%2C+anti-mouse%2C+REAfinity/pm42001515-35-4-24
Average 96 stars, based on 1 article reviews
cd43 b cells - by Bioz Stars,
2026-10
96/100 stars
Images
Related Articles
Selection:Article Title: Type I interferon promotes the fate of Toll-like receptor 9–stimulated follicular B cells to plasma cell differentiation Article Snippet: For B-cell isolation, splenic B cells were purified by negative selection of CD43 + cells with anti-CD43 magnetic beads (Miltenyi Biotec). .. Following the negative selection of Isolation:Article Title: Type I interferon promotes the fate of Toll-like receptor 9–stimulated follicular B cells to plasma cell differentiation Article Snippet: For B-cell isolation, splenic B cells were purified by negative selection of CD43 + cells with anti-CD43 magnetic beads (Miltenyi Biotec). .. Following the negative selection of Article Title: Transcriptional programming of early-forming memory B cells arises independently of cognate CD4+ T-cell interactions. Article Snippet: Mouse B-cell isolation and enrichment Splenic cell suspensions were made by mechanically forcing spleens through a 40-μm filter and lysing red blood cells with ACK lysis buffer (0.15 M NH4Cl, 10 mM KHCO3, 0.1 mM EDTA) for 3 minutes before quenching the reaction with 4 volumes of complete RPMI media: RPMI 1640 (Corning Cellgro, 50-020-PC), 10% heat-inactivated FBS (Sigma-Aldrich), 1% MEM nonessential amino acids (Sigma, ENBF3930-01), 100 μM sodium pyruvate (Sigma, RNBF6686), 10 mM HEPES (HyClone, SH30237), 1× penicillin-streptomycin-glutamine (Gibco, 10378016), and 0.0035% β-mercaptoethanol (Sigma-Aldrich). .. For adoptive transfer experiments, Purification:Article Title: Type I interferon promotes the fate of Toll-like receptor 9–stimulated follicular B cells to plasma cell differentiation Article Snippet: For B-cell isolation, splenic B cells were purified by negative selection of CD43 + cells with anti-CD43 magnetic beads (Miltenyi Biotec). .. Following the negative selection of Magnetic Beads:Article Title: Type I interferon promotes the fate of Toll-like receptor 9–stimulated follicular B cells to plasma cell differentiation Article Snippet: For B-cell isolation, splenic B cells were purified by negative selection of CD43 + cells with anti-CD43 magnetic beads (Miltenyi Biotec). .. Following the negative selection of Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion. Article Snippet: .. Class switch recombination and cell trace violet staining For the Class Switch Recombination assay, single-cell suspensions of spleen cells were sorted with CD43 magnetic beads (MACS, Miltenyi), and Staining:Article Title: Type I interferon promotes the fate of Toll-like receptor 9–stimulated follicular B cells to plasma cell differentiation Article Snippet: For B-cell isolation, splenic B cells were purified by negative selection of CD43 + cells with anti-CD43 magnetic beads (Miltenyi Biotec). .. Following the negative selection of Article Title: CRISPR/Cas9 editing reveals IRF8 regulated gene signatures restraining plasmablast differentiation Article Snippet: Isolated B cells were cultured in B cell media: RPMI 1640 (Corning Cellgro; 50-020-PC), 10% heat-inactivated FBS (Sigma-Aldrich), 10 mM HEPES (HyClone; SH30237), 1% MEM nonessential amino acids (Sigma-Aldrich; ENBF3930-01), 10 μM Sodium Pyruvate (Sigma-Aldrich; RNBF6686), 1× penicillin– streptomycin–glutamine (10378016; Life Technologies), and 0.0035% 2-mercaptoethanol (Sigma-Aldrich). .. For enrichment of adoptively transferred Thy1.1 congenically marked cells, Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion. Article Snippet: .. Class switch recombination and cell trace violet staining For the Class Switch Recombination assay, single-cell suspensions of spleen cells were sorted with CD43 magnetic beads (MACS, Miltenyi), and Recombination Assay:Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion. Article Snippet: .. Class switch recombination and cell trace violet staining For the Class Switch Recombination assay, single-cell suspensions of spleen cells were sorted with CD43 magnetic beads (MACS, Miltenyi), and Single Cell:Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion. Article Snippet: .. Class switch recombination and cell trace violet staining For the Class Switch Recombination assay, single-cell suspensions of spleen cells were sorted with CD43 magnetic beads (MACS, Miltenyi), and Magnetic Cell Separation:Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion. Article Snippet: .. Class switch recombination and cell trace violet staining For the Class Switch Recombination assay, single-cell suspensions of spleen cells were sorted with CD43 magnetic beads (MACS, Miltenyi), and Cell Culture:Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion. Article Snippet: .. Class switch recombination and cell trace violet staining For the Class Switch Recombination assay, single-cell suspensions of spleen cells were sorted with CD43 magnetic beads (MACS, Miltenyi), and Adoptive Transfer Assay:Article Title: Transcriptional programming of early-forming memory B cells arises independently of cognate CD4+ T-cell interactions. Article Snippet: Mouse B-cell isolation and enrichment Splenic cell suspensions were made by mechanically forcing spleens through a 40-μm filter and lysing red blood cells with ACK lysis buffer (0.15 M NH4Cl, 10 mM KHCO3, 0.1 mM EDTA) for 3 minutes before quenching the reaction with 4 volumes of complete RPMI media: RPMI 1640 (Corning Cellgro, 50-020-PC), 10% heat-inactivated FBS (Sigma-Aldrich), 1% MEM nonessential amino acids (Sigma, ENBF3930-01), 100 μM sodium pyruvate (Sigma, RNBF6686), 10 mM HEPES (HyClone, SH30237), 1× penicillin-streptomycin-glutamine (Gibco, 10378016), and 0.0035% β-mercaptoethanol (Sigma-Aldrich). .. For adoptive transfer experiments, Knock-Out:Article Title: Transcriptional programming of early-forming memory B cells arises independently of cognate CD4+ T-cell interactions. Article Snippet: Mouse B-cell isolation and enrichment Splenic cell suspensions were made by mechanically forcing spleens through a 40-μm filter and lysing red blood cells with ACK lysis buffer (0.15 M NH4Cl, 10 mM KHCO3, 0.1 mM EDTA) for 3 minutes before quenching the reaction with 4 volumes of complete RPMI media: RPMI 1640 (Corning Cellgro, 50-020-PC), 10% heat-inactivated FBS (Sigma-Aldrich), 1% MEM nonessential amino acids (Sigma, ENBF3930-01), 100 μM sodium pyruvate (Sigma, RNBF6686), 10 mM HEPES (HyClone, SH30237), 1× penicillin-streptomycin-glutamine (Gibco, 10378016), and 0.0035% β-mercaptoethanol (Sigma-Aldrich). .. For adoptive transfer experiments, |
![<t>Tet2</t> deficiency impairs <t>memory</t> <t>B‐cell</t> and plasma cell differentiation of IgG1+ germinal center B‐cells . (A) Schematic diagram of the protocol of SRBC primary and secondary immunizations. (B) Representative cytometry plot and quantification of IgM+ and IgG1+ GC B‐cells (CD19+CD95+GL7+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary ( n = 8) and secondary immunization ( n = 18) with SRBC. (C) Representative cytometry plot and quantification of IgM+ and IgG1+ MBCs, (Lin (CD4, CD8, Gr1)‐IgD‐GL7‐CD19+CD38+) from Tet2 ‐WT and Tet2 ‐KO mice upon primary and secondary immunization with SRBC (primary immunization: Tet2 ‐WT [ n = 7] and Tet2 ‐KO [ n = 9]; secondary immunization: Tet2 ‐WT [ n = 9] and Tet2 ‐KO [ n = 10]). (D) Representative cytometry histograms and quantification of the expression of CD80 on the cell surface of IgM+ and IgG1+ MBCs from Tet2 ‐WT ( n = 7) and Tet2 ‐KO ( n = 9) mice upon primary immunization with SRBC. (E) Representative cytometry plot of PBs <t>(CD19+CD138+CD43+)</t> from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. (F) Representative cytometry plot and quantification of IgM+ and IgG1+ PBs (CD19+CD138+CD43+) from Tet2 ‐WT ( n = 4) and Tet2 ‐KO ( n = 5) mice upon secondary immunization with SRBC. All P values were calculated using an unpaired two‐tailed t ‐test, * P < 0.05, ** P < 0.01, *** P < 0.001 and ns, not significant, in all experiments. GC, germinal center; MBC, memory B cell; PBs, plasmablasts; SRBC, sheep red blood cells.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_7087/pmc12797087/pmc12797087__HEM3-10-e70296-g006.jpg)
